Expansion of mouse sertoli cells on microcarriers

Expansion of mouse embryonic stem cells on microcarriers.

Embryonic stem (ES) cells have been shown to differentiate in vitro into a wide variety of cell types having significant potential for tissue regeneration. Therefore, the operational conditions for the ex vivo expansion and differentiation should be optimized for large-scale cultures. The expansion of mouse ES cells has been evaluated in static culture. However, in this system, culture paramete...

Comparison of Neonatal and Adult Mice-derived Sertoli Cells in Support of Expansion of Mouse Spermatogonial Stem Cells In vitro

Serum-free media for mesenchymal stem cells expansion on microcarriers

Background Expansion of mesenchymal stromal cells (MSC) is one of the key steps for their use in tissue engineering or cell therapies. To increase cells expansion yields, two milestones have to be achieved that will allow a wider MSC therapeutic use, namely an optimal serum-free medium and a process intensification via 3D suspension culture on microcarriers [1]. Indeed, one of the major obstacl...

comparison of neonatal and adult mice-derived sertoli cells in support of expansion of mouse spermatogonial stem cells in vitro

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Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells

  Objective(s): Sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. The present study was designed to analyze the effect of Sertoli cells in differentiation of mouse embryonic stem cells (mESCs) to germ cells.   Materials and Methods: A fusion construct composed of a Stra8 gene promoter and the coding region of enhanced green fluorescence p...